Purification and partial characterization of a murine melanoma-associated antigen.
نویسندگان
چکیده
منابع مشابه
Purification and partial characterization of a murine melanoma-associated antigen.
A murine B16 melanoma-associated antigen was detected and partially purified from spent culture medium and intracellular material. The antigen was also purified and partially characterized from the detergent extract of cells cultured in the presence of [3H]glucosamine done or together with [‘4C]leucine. During purification, the melanoma-associated antigen activity was monitored by a double anti...
متن کاملPartial Purification and Characterization
An enzyme fraction containing phosphatase activity for phosphorylated eukaryotic peptide initiation factor 2 (eIF-2) has been isolated from rabbit reticulocytes and partially characterized, The enzyme efficiently catalyzes release of phosphate from the small subunit of eIF-2 (eIF-2a) that has been phosphorylated by the hemin-controlled repressor. It is shown to restore activity of this phosphor...
متن کاملCharacterization and Partial Purification
A bstract. Platelets have been shown to affect the growth of vascular endothelial cells. This report describes the characterization and partial purification from human platelets of a novel growth factor which can stimulate human endothelial cells to synthesize DNA and grow. Platelets were lysed by sonication and the particulate fraction removed by ultracentrifugation at 100,000 g. The supernata...
متن کاملPurification and Characterization of Murine
The serum protein lipopolysaccharide (LPS)-binding protein (LBP) seems to play an important role in regulating host responses to LPS. Complexes of LPS and LBP form in serum and stimulate monocytes, macrophages, or polymorphonuclear leukocytes after binding to CD14. Previous reports have described the structure and properties ofLBP from human and rabbit sera. Since mice are used in some experime...
متن کاملPartial purification and characterization of cresolase and catecholase activity of black mulberrys (Morus nigra)
Polyphenol oxidase from Black mulberrys was extracted and partially purified through (NH4)2SO4 precipitation, dialysis and ion exchange chromatography. P-cresol was the better substrate for cresolase activity with a Km (11.5 mM) and catechol for catecholase activity with Km (6.4 mM) at pH 5. Km for cresolase and catecholase activity of IsoPPOII at pH 6.5 were 12 and 8.5 and for IsoPPOIII at pH ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1980
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)70762-5